Evaluation of Boar Sperm Viability by MTT Reduction Assay in Beltsville Thawing Solution Extender
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چکیده
منابع مشابه
Evaluation of Boar Sperm Viability by MTT Reduction Assay in Beltsville Thawing Solution Extender*
MTT (3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) reduction assay is a method that validates the viability of an active cell. Dehydrogenase in mitochondria converts yellow colored insoluble tetrazolium salt to purple colored watersoluble formazan. Sperm also have mitochondria in the midpiece, therefore sperm viability could be evaluated by MTT reduction assay. Several studi...
متن کاملAssessment of bovine sperm viability by MTT reduction assay.
The MTT reduction assay depends on the ability of metabolically active cells to reduce the tetrazolium salt (3[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide) to formazan. This study was conducted to examine and validate a simple and less costly MTT test to determine bovine sperm viability and compare the efficiency of this test with a flow cytometer. Fresh ejaculates from eight bull...
متن کاملsperm mtt viability assay: a new method for evaluation of sperm viability
introduction: sperm viability test is important in diagnosis and treatment of male infertility. the common test is e&n; and host which they are based on membrane permeability and integrity. the aim of this study was to evaluate mtt assay as a sperm viability test based on mitochondrial activity. materials and methods: washed sperm samples were co-incubated with mtt in different media and differ...
متن کاملMicroscopic analysis of MTT stained boar sperm cells
The ability of sperm cells to develop colored formazan by reduction of MTT was used earlier to develop a spectrophotometric assay to determine the viability of sperm cells for several mammalian species. It was the objective of the present study to visualize microscopically the location of the formazan in boar sperm cells. The MTT staining process of boar sperm cells can be divided into a series...
متن کاملP-55: Cryopreservation of Rooster Semen Using Hand-Made Cryopreservation Media Supplemented with Different Cryoprotectants
Background Cryopreservation procedure for poultry semen is not reliable due to structural and biochemical damages which can lead to reduction in sperm quality and fertility. Development of cryopreservation medium has crucial effects for successful recovery of sperm after freezing. The objective of this study was assessment of cryopreservation of rooster semen using hand-made cryopreservation me...
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ژورنال
عنوان ژورنال: Asian-Australasian Journal of Animal Sciences
سال: 2008
ISSN: 1011-2367,1976-5517
DOI: 10.5713/ajas.2008.70480